Snodgrassella alvi wkB2, tn-SEQ experimental library replicate 3 - SRA

SRX1725576: Snodgrassella alvi wkB2, tn-SEQ experimental library replicate 3
1 ILLUMINA (Illumina MiSeq) run: 3.5M spots, 519.2M bases, 295.3Mb downloads

Design: tn-SEQ library of Snodgrassella alvi strain wkB2 (bearing a gentR cassette from the Mariner transposon from pBT20) recovered from dissected ileums of 50 Apis mellifera individuals after 5 days post inoculation. gDNA was isolated via a CTAB/bead beating method, fragmented to ~ 300 bp used as a template for C-tailing via TdT. Transposon junctions in C-tailed template were amplified with a transposon specific primer paired with a poly G biotinylated primer. Amplicons bearing insertion junctions were magnetically pulled down, barcoded and adapted for illumina sequencing via PCR. Amplicon was sent for sequencing at the University of Texas at Austin GSAF.

Submitted by: University of Texas at Austin

Study: Snodgrassella alvi wkB2 Tn-SEQ and RNA-seq raw sequence reads

PRJNA319191 • SRP073800 • All experiments • All runs

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Read data from Tn-SEQ and RNA-seq of Snodgrassella alvi strain wkB2 from control replicates of plated bacteria and from replicates of pooled ileums of inoculated Apis mellifera workers

Sample: Microbe sample from Snodgrassella alvi wkB2, tn-SEQ experimental library replicate 3

SAMN04851111 • SRS1411206 • All experiments • All runs

Organism: Snodgrassella alvi wkB2

Library:

Name: EXP3

Instrument: Illumina MiSeq

Strategy: Tn-Seq

Source: GENOMIC

Selection: PCR

Layout: SINGLE

Spot descriptor:

1 forward

Runs: 1 run, 3.5M spots, 519.2M bases, 295.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR3438432	3,461,575	519.2M	295.3Mb	2016-11-16

ID:: 2470023

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Sequence Read Archive

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